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Class 4 Laser Science & Studies

Mechanistic approach to GaAIAs diode laser effects on production of reactive oxygen species from human neutrophils as a model for therapeutic modality at cellular level

Makoto Yamaya*, Chiyuki Shiroto’, Hiroki Kobayashi*, Shinji Naganuma*, Jyuichi Sakamoto*, Koh-Jun Suzuki*, Shigeyuki Nakaji*, Kazuo Sugawara* and Takashi Kumae *Department of’ Hygiene, Hirosaki University School of Medicine. Hirosaki; .-Shiroto Clinic Coshogawara, Aomori: Department of Industrial Health. The Institute of Public Health, Tokyo. Japan.

There have been many reports on the applications of low reactive level laser (LLL) therapy for pain attenuation or pain removal. Our group has reported previously on the effects of in vitro irradiation of LLLT particularly on the phagocytic activity of human Neutrophils, using luminol-dependent chemiluminescence (LmCL) for measurement of reactive oxygen species (ROS) production from human Neutrophils. However, the mechanisms of the attenuation of phagocytic activity of NEUTROPHILS by LLL irradiation is not yet full understood.

In this study. we used luminol-dependent and lucigenin-dependent chemiluminescence (LgCL) for detection of affected ROS producing process of human Neutrophils by LLL irradiation. Two soluble action stimuli, N-formyl-Met-Leu-Phc (fMLP) and phorbol myristate acetate (PMA) were used to avoid the possible influence of lag-time from recognition to uptake of particles at the ROS production.

In case of using fMLP as a stimulus, the maximum luminescence intensity of LULL was increased hut LgCL luminescence was decreased by LLL irradiation. When PMA was used as a stimulus, the times to reach the maximum luminescence intensity of LmCL and LgCL were shortened by LLL irradiation, but there was no effect on the maximum luminescence intensity of both.

These results suggest that LLL irradiation enhances the ROS production activity of human Neutrophils by the activation of the superoxide converting system, the active clement in which is mainly myeloperoxidase. LLL irradiation enabled a more rapid activation of the superoxide production system, NADPH -oxidase. 

Laser Therapy Science and Clinical Studies

CLASS IV LASER THERAPY – AKA PHOTOMEDICINE IS DELIVERING MANY BENEFITS THROUGH INTEGRATIVE MEDICINE PRACTITIONERS Clinical Results Reveal Many Transformative and Encouraging Benefits Such As: 1. Anti-Inflammatory EffectEffect of Laser Therapy on the Expression of Inflammatory Mediators… Pubmed Laser Therapy reduces inflammation with vasodilation, activation of the lymphatic drainage system, and reduction of pro-inflammatory mediators; as a result, inflammation, erythema, bruising, and edema are reduced. This is one of the primary goals in Photomedicine (Laser)

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Laser Therapy and Musculoskeletal Pain Syndromes (Chronic and Acute)

Musculoskeletal Pain Syndromes (Chronic and Acute) Low-Level Light Therapy (LLLT) has been shown to be effective in a variety of musculoskeletal conditions and associated pain presentations. In Rheumatoid Arthritis, LLLT can benefit not only the pain of acute small joint inflammation but also chronic pain. In a review article on rheumatology (3), some 18 papers were considered. All studies involved double-blind trials with LLLT in chronic rheumatoid and reported significant improvement in pain (80% success

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Acute Respiratory Distress Disorder Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Acute Respiratory Distress Disorder. These studies are presented here to demonstrate the wide uses of cold lasers in the treatment of different medical conditions. Acute Respiratory Distress Syndrome Successfully Treated with Low-Level Laser Therapy Chan Gunn, Institute for the Study and Treatment of Pain Abstract Acute respiratory distress syndrome (ARDS) is a medical emergency, which may be

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Alcoholism Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Alcoholism. These studies are presented here to demonstrate the wide uses of cold lasers in the treatment of different medical conditions. Acupuncture for alcohol withdrawal: a randomized controlled trial 1 Psychiatric Hospital,2 Department of Complementary Medicine (KIKOM), Inselspital, University of Berne, 3010 Berne, 3 Department of Social and Community Psychiatry, University of Berne, Laupenstrasse 49, 3010 Berne, Switzerland,

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Alzheimer’s Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using a cold laser to treat Alzheimer’s Syndrome. These studies are presented here to demonstrate the wide uses of a cold laser in the treatment of different medical conditions. The Efficacy of 904 nm Laser Therapy for Alzheimer’s Diseases Kazuyoshi Zenba, Vice president of Kanagawa Acupuncture Massage Association Prof. Masayuki Inoue, Secretary of JLPLTPA Preface: Although we had reported about the possible

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Laser Acupuncture for Treating Periarthritis Humeroscapularis

Branka Nikolic,. spec. Sport’s med. Address Zavod za zdravstvenu zastitu radnika ZTP-a “Beograd“, 11000 Beograd, Slobodana Penezica Dr. 23 Yugoslavia The effect of low intensity semi conductor laser was used as treatment methods for periarthritis humeroscapularis. The CC laser (Computer Controlled laser) was applied. Laser therapy has positive biological effects and antiinflamatory, antioedema effects and analgesia. We treated 18 patients with periarthritis humeroscapularis, 14 were female patients. The laser was locally applied at the AC points Sj 14, Sj 15, Li 15, Li 10, Sj 5, Si

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Arteriosclerosis Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Arteriosclerosis. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. Low Level Laser Therapy in the Treatment of Arteriosclerosis of the Lower Limbs Attia M.A., EI-Kashef H. Laser center, Alhikmah Hospital, EI-Mansoura, Egypt Physics Department, Faculty of Science, Tanta Egypt Abstract: Twenty patients with arteriosclerosis

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Arthritis-Cervical Cold Laser Clinical Studies

The following is a summary of some of the clinical studies that were conducted using cold laser to treat Arthritis-Cervical. These studies are presented here to demonstrate the wide uses of a cold lasers in the treatment of different medical conditions. The clinical efficacy of low-power laser therapy (lllt) on pain and function in cervical osteoarthritis. Ozdemir F, Birtane M, Kokino S. Department of Physical Therapy and Rehabilitation, Medical Faculty of Trakya University, Edirne, Turkey.Clin

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Guide to Class 4 Therapy Lasers

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Guide to Class 4 Therapy Lasers

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